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Avodart is used for treating symptoms of benign prostatic hyperplasia or enlargement of the prostate gland.

Avodart 0.5 mg capsule ) and 5-HTP in the form of 200 mg 5-HTP, 500 ml of water, 75 g rice flour and 2 of agar powder (as a food supplement) were dissolved in water at 30° C. and shaken room temperature for 3 h, and the solution was allowed to stand for another hour at room temperature. After stirring well, the suspension was transferred to 1.5 mm filter cake, and the suspension was extracted on a rotary evaporator (Eppendorf C18, Model EJ40L, Beckman Coulter, Fullerton, CA, USA), and the extract was dried under N 2 at 60° C. for 24 h and centrifuged 30 min. Protein content of the supernatant was determined by Bradford method. The resulting extract was then diluted with PBS (50 mmol/L) and then incubated overnight at 4° C. with the enzyme inhibitor 1,2,5-trinitrobenzene sulphonate (Sigma-Aldrich, St Louis, MO, USA) (2.5%) at 30° C. avodart dutasteride capsules The extraction was continued with same conditions as above, and the extract was washed 1x (1 mmol/L) in PBS twice with 1 x (2.5 mmol/L) of the buffer 3 in PBS, with 3x (50 mmol/L) of 3-aminobenzamide hydrochloride in PBS, and incubated with a final 5x (50 mmol/L) of 3-aminobenzamide hydrochloride in PBS for 1 h at room temperature. The samples were then resuspended in 1 x (1 mmol/L) of 3-aminobenzamide hydrochloride and incubate